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[期刊论文] 肖艳 杨易
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EI CSTPCD 北大核心
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摘要:该文利用基于有限面元(Finite Area Element,FAE)方法对拱形屋面的不均匀积雪荷载进行了研究.首先设计制作了缩尺比为1:100、矢跨比为0.125的拱形屋面风洞试验模型,通过风洞试验测得屋面平均风速大小,结合CFD数值模拟确定平均风速方向,再基于FAE方法利用风速-雪通量经验公式计算得到屋面的积雪分布系数;并分析了不同风速和不同风向角对屋面积雪分布系数及不平衡雪荷载的影响.其次,对基于FAE方法计算得到的积雪分布系数、基于两相流的CFD数值模拟结果以及我国现行《建筑结构荷载规范》GB 50009-2012的结果进行了比较.最后,总结了屋面雪荷载分布规律,给出了针对这类大跨结构雪荷载设计的一些建议.
摘要:目的:观察抑制beclin-1对神经元缺血再灌注损伤和细胞自噬变化。
方法:体外培养N2a细胞,脂质体转染beclin-1的miRNA干扰质粒,以缺氧缺营养方式模拟缺血再灌注,MTT法检测各组细胞活性,Western lotting检测beclin-1、LC3、Caspase-3表达,电镜观察细胞自噬活性。
结果:各治疗组beclin-1的表达均比假治疗组有显著差异,LC3的表达两组之间并无差异;在缺血90分钟再灌24小时治疗组细胞活性比假治疗组明显升高;治疗组Caspase-3的表达比假治疗组偏低,差异有显著意义,两者之间LC3表达无明显差异;而在缺血30分钟再灌24小时细胞活性、Caspase-3和LC3的表达比真假治疗组无明显差异。
结论:在较大的持续应激时,beclirrl的高表达并不有利细胞存活,干扰beclin-1的表达虽不能明显改变自噬水平,却减少了凋亡,有利细胞存活。
摘要:公积金这种政策性的金融制度起源于新加波,经过数十年的发展,已成为新加坡金融系统中重要的组成部分.我国从20世纪90年代开始借鉴新加坡的公积金制度创建了我国的住房公积金制度,这种制度对我国住房产业的发展起到一定的支撑作用.文章旨在通过对新加坡公积金制度的全面了解和剖析,采提炼出有利于我国住房公积金制度的经验.
[硕士论文] 杨易
生物化学与分子生物学 华中科技大学 2011(学位年度)
摘要:Multiple cell signaling pathways and biological processes are involved in cerebralischemia/reperfusion (I/R)injury,among which,autophagy is a most confusmg one.Studiessuggest that autophagy is activated during ischemia and reperfusion (I/R),however there'sno data to dynamically mimic autophagy level during the whole process of I/R,and it's stillnot known whether autophagy functions as a guardian or plays a detrimental role in I/RinJury. Here we employed simulated I/R of N2a cells (mouse neuroblastoma cells)as an in vitromodel of I/R injury to the neurons.LC-311 level was checked by immunoblot analysis atdifferent time points of ischemia process,and different intervals of reperfusion.Resultsshowed that autophagy was gradually activated as ischemia went on,and greatly elevatedduring the process of reperfusion after 90minutes' ischemia insult.I/R manipulation wasalso applied to N2a cells transfected with EGFP-LC3,punctate EGFP-LC3 was monitored,percentage of cells displaying obvious EGFP-LC3 puncta coincided with the forenamedimmunoblot results.Also,we found that autophagy was induced in hippocampus andcortex of rats underwent MCAO for 90 minutes and reperfusion for a week. Beclin-1 in cytosol,p-mTOR,p-P70 S6K were also checked in ischemia/reperfusiongroups by immunoblot analysis,results indicated that Beclin-1 in cytosol shared the samechange pattern with LC-311,while p-P70 S6K and p-mTOR showed delayed decrease afterreperfusion.Administration of 3MA,inhibitor of class Ⅲ PI3K,definitely abolishedautophagy during reperfusion,while employment of rapamycin,inhibitor of mTORC1,surprisingly weakened autophagy activation during reperfusion. Analyses of mitochondria function by relative cell viability were performed in I/R modelgroups and 3MA-treated groups,results came that autophagy inhibition by 3MA definitelyhold back the decline of mitochondria function during the process of reperfusion.
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