流式细胞术分析强声波对植物细胞周期的影响

李涛　 侯月霞　 蔡国友　 沈子威　 席葆树　 陶祖莱　

摘　要：应用流式细胞术分析烟草细胞在交变应力作用下细胞周期的变化。用特制的强声波发生装置产生频率和强度可调的交变应力场，研究不同频率和强度的交变应力作用后烟草细胞周期的变化。实验结果表明，在交变应力作用下直接影响细胞或细胞分裂的同步化，促进S期的DNA合成，有助于细胞有丝分裂。声波频率在400Hz至800Hz，强度在90dB到110dB内，随频率和强度的增加，交变应力使S期细胞明显增加，但频率或强度过大，反而使S期细胞大大减少。
关键词：流式细胞术（FCM）；交变应力；细胞周期；烟草细胞
分类号：Q689　文献标识码：A
文章编号：1000－6737(2001)01－0195－04

ANALYSIS OF THE EFFECT OF STRONG SOUND WAVE ON PLANT CELLS CYCLES USING FLOW CYTOMETRY

LI Tao（Institute of Mechanics,Chinese Acadmy of Science,Beijing 100080,China）　
HOU Yue-Xia（Department of Applied Chemistry,China Agricultural University,Beijing 100094,China）　
CAI Guo-You（Department of Biological Science and Biotechnology,Tsinghua University,Beijing 100084,China）　
XI Bao-Shu（Department of Mechanics,Tsinghua University,Beijing 100084,China）　
TAO Zu-Lai（Institute of Mechanics,Chinese Acadmy of Science,Beijing 100080,China）　

Abstract：Flow cytometry was used to measure cell cycle parameters in tobacco cell suspensions acted by alternative stress.The field of alternative stress was generated through a strong sound filed system.It was found that the effects of stress on cells depended greatly on the intensity and frequency of stress.In a certain range of intensity and frequency,the stress caused a significant changes to cell cycle paraments and an increase of the proportion of cells in S phase was seen,while the stress with higher frequency or intensity may cause the proportion of cells in S phase decreased evidently.
Keywords：Flow Cytometry (FCM); Alternative stress; Cell cycle; Tobacco cell

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收稿日期：2000年8月25日

出版日期：2001年3月1日