The cytotoxic activity of a novel ether-linkaged bisalkaloid isolated from the root of Thalictrum acutifolium (Hand.-Mazz.) Boivin., acutiaporberine, on several human carcinoma cell lines was described for the first time. Acutiaporberine was added to the culture in 7 concentrations ranging from 2.5μg/mL to 100 μg/mL. It showed cytotoxic activity to four cultured human carcinoma cell lines, i.e. Hela (IC50 = 37 μg/mL), LCC (IC5o = 60 μg/mL), MGC (IC50 = 65 μg/mL), NSCLC (IC50 = 82 μg/mL), and two normal cell lines, L-02 (IC50 = 65 μg/mL) and NIH 3T3 (IC50 = 78 μg/mL).But it was less active to HepG2 and Mcf- 7 (IC50＞ 100μg/mL).