Objective To explore the HIV and syphilis infection and AIDS-related behaviors among money boys (MB) in Guangzhou. Methods A total of 152 subjects were recruited from MB gathering place (clubs, parks, internet etc. )by a local NGO. Of which, 151 individuals completed the investigation and blood sample collection. An anonymous face to face interview were used to obtain data of AIDS-related behavior,knowledge and attitudes. Venous blood samples were collected for HIV and syphilis antibody test. x2 test were used to compare the characteristics of HIV and syphilis infection subjects and P ＜ 0. 05 was counted as significant. Results HIV and current syphilis infection rate were 11.3% ( 17/151 )and 19. 9% (30/151),respectively. Among the subjects,47.0% (71/151)had unprotected anal intercourse(UAI) with commercial male sex partners, 43. 7% (66/151) had UAI with non-commercial male sex partners in the past six month. 85.4% ( 129/151 )were aware of AIDS-related knowledge, while 34.4% (52/151) thought they have no risk of HIV infection,24.5% (37/151)didn't know the prevalence of HIV among MSM in China, and 55.0% (83/151) had no idea of HIV prevalence or thought HIV prevalence was not serious at all among MB. MB who had experienced sex after drunk/ used drugs had higher proportion of syphilis infection (33.3% vs 16. 5%; x2 = 4. 26, P = 0. 039), and who had ever been experiencing condom broken during sex had much higher syphilis infection rate than those didn't ( 36. 7% vs 15.7%; x2 = 6. 64, P = 0. 010). A multi-Logistics regression analysis showed that subjects had ever been experiencing condom broke during sex in the past six month were associated with syphilis infection (x2 = 6. 24, P = 0. 012; OR = 3. 11,95% CI=1.28 -7. 57). Conclusion Money boys have high prevalence of HIV and syphilis infection. They are lack of perception of HIV risk and active in unsafe sex behavior.