Objective To study the effect of glucocorticoids in treatment with the epidemic keratoconjunctivitis. Methods All 480 patients with epidemic keratoconjunctivitis were collected in our hospital from 2002 to 2007 and randomly divided into four groups. The first group did not use glucocorticoids; the second group started using glucocorticoids from the very beginning of the treatment; the third group started using glucocorticoids when punctate epithelial corneal opacity and fluorescein staining positive; the fourth group started using glucocorticoids until the appearance of the white round subepithelial corneal opacity. Results 80% of the first group appears punctate epithelial corneal opacity and left white round subepithelial corneal opacity in the end; the second group showed no keratopathy occurred; 21% of the third group and all the patients in the fourth group of all patients left white round subepithelial corneal opacity in the end. Conclusions Using glucocorticoids timely is an effective and safe treatment in patients with epidemic keratoconjunctivitis.