The effect of high level copper diets on mitochondrial permeability of liver, lipid metabolism,liver and mus-cle copper content in broiler was investigated in this study. 120 l-day-old healthy AA broiler were divided randomly into four groups. Based on the control group (11 mg/kg Cu), three experimental groups were set up, they were 110, 220,330 mg/kg Cu, respectively,all of the experimental broilers were fed with corn-soybean meal containing copper as copper sulfate for 60 days. The results showed that:(1) Mitochondrial permeability transition pore (MPTP) grad-ually enlarged (P<0.05) along with the increase of copper level and/or days broilers raised. (2) There was a de-crease (P>0.05) in serum total cholesterol with no significant difference between the control and 110,220 mg/kg supplemental level,serum total cholesterol at 330 mg/kg significantly decreased (P<0.01) compared to the control. There was a decrease (P<0.01) in serum TG with significant difference between the control and 110,220,330 mg/kg. (3) The accumulation of Cu in the liver increased (P>0.05) at 110 mg/kg and 220 mg/kg supplemental level and significantly increased (P<0.01) at 330 mg/kg in the control. Accumulation of Cu in the muscle increased sig-nificantly (P<0.01) at all supplemental levels. The results demonstrated that high level copper diets could result in the swell and injury of mitochondria to some degree,alter lipid metabolism in broilers and obviously increase the ac-cumulation of Cu in the liver and muscle.