The main purpose of this paper is to mutant Agrobacterium sp. DH02 and screen for stain with high yield of curdlan. A high-yield strain 39# was successfully selected by comparing the crude yield, refined yield and gel strength of curdlan. Its crude yield was 30.5 g/L, while the yield of parental strain DH02 was 25.1 g/L, increased by 21.5%. Gel strength was respectively 811.12 g/cm2 and 593 g/cm2 for strain 39# and DH02.