Objective To observe effects of tetrahydroxystilbene glucoside ( TSG) on behavior and content of dopamine and its metabolites in striatum of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP) model mice. Methods Mice were randomly divided into normal control group, model group, and TSG low dose(60 mg/ kg) and high dose(120 mg/ kg) groups. The behavior changes of mice were observed by pole test, Rotarod test and spontaneous movement test. The tyrosine hydroxylase(TH) positive cells were detected by immunohistochemical method. The content of dopamine(DA) and its metabolic products in striatum were determined by HPLC-ECD. Results MPTP model mice showed behavior deficit. The counts of TH positive neurons in substantia nigra and the content of dopamine and its metabolites in striatum in model mice decreased significantly compared with control group. TSG ameliorated the behavior deficits, increased the number of TH positive neurons in the substantia nigra, and elevated the content of dopamine in striatum compared with model mice. Conclusion TSG protected dopaminergic neurons against MPTP-induced damage, and may become a candidate drug for prevention and treatment of Parkinson's disease.