Appropriability of technical innovations is crucial to the development of the company. With the development of the economic globalization, appropriability is confronted with more challenges. As the world mostly economic force, the empirical practice of multinational companies (MNCs) is very important for the Chinese enterprises which will become more internationalization to learn form it. Based on the multinational innovation system, this paper builds up the new concept framework on the appropriability from the R&D regional organization of the MNCs in the world. Based on statistical analysis of the 120 subsidiaries of MNCs in China on the use of non-legal mechanism through questionnaires, this paper tests the hypothesis and demonstrates that MNCs use more non-legal mechanism to protect their explicit knowledge, and the R&D regional organization of the MNCs is very important for the selection of the mechanism of appropriability.