Objective: To investigate the role of curcumin in cisplatin induced apoptosis of human gastric cancer HGC27 cells. Methods: Cytotoxicity of cisplatin and curcumin were detected by CCK-8 assay. The amount of apoptotic cells was detected by hochest 33258 staining assay. The DNA damage biomarker p-γH2AX and cell apoptosis biomarker cleaved-PARP1 were detected by Western blot. Results:Cisplatin induced HGC27 cells death in a dose-dependent manner. The proliferation of HGC27 cells was not affected by curcumin at 5μmol·L-1, lightly enhanced at 10μmol·L-1, while inhibited at 20, 40 and 80μmol·L-1 for 10.97%, 15.15 % and 32.93 %, respectively. The combination of 5 or 10μmol·L-1 curcumin with different concentration of cisplatin could not induce HGC27 appoptosis effectively (P>0.05). However, significantly increased cell death was observed in HGC27 cells with the combination of 20 μmol·L -1 curcumin and 4 or 6 μmol ·L -1 cisplatin. Hoechst33258 staining showed that apoptotic cells were significantly increased in the combined group. Western blot results also showed that the levels of DNA damage marker p-γH2AX and apoptosis marker cleaved-PARP1 were significantly increased in the combined group. Conclusion: Curcumin enhances cisplatin induced apoptosis by inhibiting DNA repair in human gastric cancer HGC27 cells.