Objective: To label JWA monoclonal antibody with horseradish peroxidase (HRP) and apply it in ELISA and western blot immune detection. Methods: Using sodium periodate and sodium borohydride redox reaction, HRP was activated; The activated HRP was used to mark JWA monoclonal antibody in tubes; The HRP labeled antibody was respectively applied in direct, sandwich ELISA and western blot de-tection. Results: HRP was successfully labeled on JWA monoclonal antibodies. In the direct method of ELISA test, HRP-JWA (4c9) antibodies combined polypeptide better than the combination of HRP-JWA (7c3); Its max OD450 was 0.96. While in double sandwich ELISA experiment, pre-coated by JWA (4c9) an-tibody, using the HRP-JWA (7c3) antibody as detected antibody could get good results, the max OD450 was 1.30. HRP-JWA could also identify the target protein in SGC7901 cells in western blot experiments. Conclusion: JWA antibody has been successfully marked with HRP, which could be used preliminarily in ELISA and western blot.