In order to find out micro-spore extracting method suitable for transcriptome sequencing,this study took so-matic embryos of Lonicera macranthoides Hand.-Mazz.as experimental material and micro-spore induced with globular embryo,heart shaped embryo,torpedo embryo and embryo as research object.This study compared and analyzed the RNA extraction effects by 3 different methods:EASYspin Plus method,RNA Cleanup Kit method,improved CTAB method.The results showed that RNA concentrations extracted by improved CTAB method were lower,and degradation-appearedinextraction process.Integrity of RNA extracted by RNA Cleanup Kit method was bad,and the RIN value did not meet the requirements of transcriptome sequencing.Quality of RNA extracted by EASYspin Plus method was the highest,and the RNA integrity was also good.Therefore,RNA obtained by this method was suitable for satisfying the requirement of transcriptome sequencing.