Objective To explore effects of Tongxinluo on hemorheology and PAI level in rats with type 2 diabetic nephropathy. Methods The rat model of type 2 diabetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet. Diabetic rats were randomly divided the model group (n=10) and the Tongxinluo group(n=10). Normal SD rats (n=10) were used as the control group. After intervention with Tongxinluo for 8 weeks, fasting blood glucose (FBG), serum creatinine (Scr), serum urea nitrogen (BUN),tissue plasminogen activator (t-PA), plasminogen activator inhibitor type-1 (PAI-1) and hemorheology indexes were detected. Results Compared with control group, FBG, SCr, BUN, hemorheology indexes in the model group were significantly increased while the PAI-1 was decreased (P＜0. 05). Compared with model group, BUN and Scr were significantly decreased (P＜0. 05),hemorheology indexes and PAI-1 levels were significantly decreased(P＜0.05),but the FBG and tPA level had no obvious influence. Conclusion TongXinLuo can protect renal function and alleviate renal pathological damage in type 2 diabetic nephropathy rats. Anticoagulation, defibringen and improving local microcirculation perfusion may be the mechanisms of delaying DN progression.