Objective To investigate the effect of dexmedetomidine on inflammatory cytokines in pa-tients undergoing laparoscopic surgery. Methods Fifty ASAⅠ~Ⅱpatients aged 40-62 yr weighing 40-72 kg scheduled for laparoscopic total hysterectomy were randomly divided into 2 groups(n=25):control group and dexmedetomidine group. D Dexmedetomidine 1. 0 μg·kg-1 was infused iv over 10 min before anesthesia induction ,and then was infused at a rate of 0. 5μg·kg-1·h-1(group D) until 30 min bero-re the end of operation. Group C received the equal volume of normal saline. Blood samples were collect-ed T0 ( before anesthesia induction ) , T1 ( 30 min after pneumoperitoneum ) , T2 ( at the time of closing pneumoperitoneum ) and T3 (4 hours after operation) for determination of serum CRP,TNF-α and IL-1βconcentrations by nephelometry and ELISA. Results Compared with T0, serum CRP,TNF-α and IL-1βconcentrations were significantly increased at T1 ~T3 in group C and at T2 ~T3 in group D( P<0. 05 or 0. 01). Compared with group C, serum CRP,TNF-α and IL-1β concentrations were significantly de-creased at T2 ~T3 in group D(P<0. 05 o). Conclusion Dexmedetomidine 1. 0 μg·kg-1 given before induction and then infused at a rate of 0. 5 μg·kg-1 ·h-1 during operation can decrease the concentra-tions of serum Inflammatory cytokines such as CRP,TNF-αand IL-1βin patients undergoing laparoscopic surgery.