Objective To understand the detection situation and epidemiological characteristics of diarrheogen-ic Escherichia coli (DEC) in Huaian from 2013 to 2015, so as to provide evidence basis for prevention and treatment of disease. Methods A total of 795 stool specimens were isolated from patients in sentinel hospitals and community hospi-tals of Huaian from 2013 to 2015 for detection of Escherichia coli. Diarrheogenic E. coli strains were screened by multi-ple PCR and verified by biochemical identification. Results A total of 80 diarrheogenic Escherichia coli strains were isolated, with a detection rate of 10.06%, including 34 strains of enterotoxigenic E. coli (ETEC), 18 strains of enteroag-gregative E. coli (EAEC), 16 strains of enteropathogenic E. coli (EPEC), 7 strains of enteroinvasive E. coli (EIEC) and 5 strains of enterohemorrhage E. coli (EHEC). There were statistical differences (P<0.05) between seasons, and the posi-tive detection rate in autumn and summer were higher. There was no significant difference between urban and rural areas (P>0.05). There was no significant difference in the positive detection rates between different genders from the popula-tion distribution analysis (P>0.05), but there were significant differences between the different age groups (P<0.05). Conclusion ETEC is the predominant type of diarrheogenil E. coli in Huaian. The detection rates are higher in summer and autumn, and also in patients aged ≤5 years and 6~18 years. Monitoring of key periods and population should be strengthened in the future.