Fourier transform infrared spectra (FTIR) of royal jelly (RJ) stored at different temperature and after different stor-age periods were measured, a series of correlation analysis among the spectra were carried out by using the spectra of new-har-vested RJ as a standard. The results showed that the correlation coefficient of amidc band I and the relative intensity ratios of I_(1647)/I_(1541), I_(1647)/I_(1409), I_1647/I_1247 and I_(1647)/I_(1054) of RJ samples' spectra decreased with extension of storage time and tempera-ture, and existed good linear correlations with the storage time, with the order of their change extent being 28 ℃＞16 ℃＞4 ℃＞-18℃. According to the spectra change laws and practical experiences of RJ storage, the correlation coefficient of amide band I and four relative intensity ratios I_(1647)/I_(1541), I_(1647)/I_(1409), I_(1647)/I_(1247) and I_(1647) /I_(1054) were selected as assessment indexes of RJ freshness. The threshold value of correlation coefficient was set to be 0. 910 0, and the threshold values of the four relative intensity ratios were set to be 1. 744, 2. 430, 3. 345 and 1. 412 respectively. Once one or more indexes are lower than the corre-sponding threshold values, the RJ sample can be considered as a stale sample. So, FTIR spectroscopy combined with several da-ta-processing methods would be an effective method for overall assessing the freshness of RJ.