[Objective] The research aimed to compare the distribution of berberine hydrochloride in the stem, leaf, root and seed of Oxytropis myriophy by two different ultrasonic extraction techniques.[ Method] Two kinds of ultrasonic extraction process were used to extract and optimize the berberine hydrochloride in the stems, leaves, roots and seeds of Oxytropis myriophy(The first technology was to extract the Mongolian medicine first by ultrasonic extraction and then placed 24 h, and the second technology was to put the Mongolian medicine at room temperature for 24 h before ultrasonic extraction.).And calculated the content of berberine hydrochloride and recovery in 426 nm wavelength spectrophotometry.[Result]The content of berberine hydrochloride was the most in the stalk of Oxytropis myriophy, the distribution of berberine hydrochloride in leaves, roots and seeds was obviously reduced,and the second technology of extraction process was better than the first technology , the recovery rate was 92.58％-110.00％, the relative standard deviation (RSD) was 0.7823％-0.9749％.[Conclusion]The distribution of berberine hydrochloride is not equal in the medicinal parts of Oxytropis myriophy, and the distribution in the stems is much more significantly, and the second technology is better than the first in the two different ultrasonic extraction techniques .