Aimed to discuss the differences genetic background between main cultivated and fresh-eating Canarium album （Lour.）Raeusch ISSR-PCR markers were used to analyze the genetic diversity of 19 Canarium album （Lour.）Raeusch cuhivars. A total of 8 ISSR primers selected from 40 primers were used to amplify 110 DNA fragments and of which 78 （70.91% ）were polymorphic.The average number of DNA bands amplified by each primer was 14. Based on SPSS 13.0 analysis,tbe similarity coefficients between different cuhivars ranged from 0.429 to 0.873 ,and the average genetic similarity coefficients was 0.656. The dendrogram constructed by UPGMA ,which genetic distance was 0.635, showed that 19 Canarium album （Lout.） Raeusch cultivars were classified into four groups, Hangweiliuhao and Makengshihao made up of the first group, Minqingqihao was the second group, minqingbahao was the third group,and the others constituted the fourth group. The study will provide the reference for utilization and new variety selection of Canarium album （ Lour. ） Raeusch resources.