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Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer

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Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys. For SCNT using fetal monkey fibroblasts, 6 pregnancies were confirmed in 21 surrogates and yielded 2 healthy babies. For SCNT using adult monkey cumulus cells, 22 pregnancies were confirmed in 42 surrogates and yielded 2 babies that were short-lived. In both cases, genetic analyses confirmed that the nuclear DNA and mitochondria DNA of the monkey offspring originated from the nucleus donor cell and the oocyte donor monkey, respectively. Thus, cloning macaque monkeys by SCNT is feasible using fetal fibroblasts.

摘要： Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cyn... 查看全部>>

作者：

Liu, Zhen Zhang, Chenchen Poo, Muming Cai, Yijun Lu, Yong Xu, Yuting Wang, Yan Nie, Yanhong Zhang, Xiaotong Wang, Zhanyang Sun, Qiang
作者单位：

Chinese Acad Sci, Inst Neurosci, CAS Ctr Excellence Brain Sci & Intelligence Techn, CAS Key Lab Primate Neurobiol,State Key Lab Neuro, Shanghai, Peoples R China
刊名：

Cell
年，卷(期)：
2018,172(4)
分类号：
Q2
页数：

共1页
页码：

881-+